Soung Hoon Chang has completed his MD at the age of 25 years from Korea University College of Medicine and has completed PhD at the age of 40 from Korea University College of Medicine. He is the director of Department of Preventive Medicine, Konkuk University School of Medicine. He has published more than 200 papers in reputed journals.
This study aims to describe the epidemiology of influenza-like illness (ILI) and to estimate the incidence rate of ILI in several communities in South Korea, over three influenza seasons. Since 2011, the Community Respiratory Health Survey has been continuously monitoring ILI in 3 community-based cohorts. At the beginning of the season, baseline study took place; Nov. 2011 in Chungju and Ulsan, and in Dec. 2012 in Seoul. For each cohort, approximately 1,000 individuals (age 6 months over) from randomly selected 300 households were recruited. On a weekly basis, participants were asked via telephone interview whether they had ARI (acute respiratory illness) or ILI; from May to October, interview took place every second week. The annual Age-standardized rate (ASR) of ILI per 1,000 populations were 2.3 (95% CI 1.8-2.9) and 3.5 (95% CI 2.7-4.3) in Chungju and Ulsan, 2011/2012 season. In 2012/2013 season, ILI incidence rate were 3.0 (95% CI 2.9-3.1) and 5.5(5.4-5.7) in Chungju and Ulsan. The ASR of ILI per 1,000 populations in Seoul was 12.7 (95% CI 12.5-12.9) per 1,000 populations in 2012/2013. From 44th week 2013 to 29th week 2014, the incidence rates were almost doubled in Seoul and Chungju due to the epidemic of influenza. In Chungju and Ulsan, ILI incidence rates were 4.3(95% CI 4.2-4.5) and 9.0 (95% CI 8.8-9.2) per 1,000 populations. In Seoul, ILI incidence rate of the 2013 season was 21.2 (95% CI 20.9-21.5) per 1,000 populations. The influenza-like illness incidence rate is highest in age 0-6 years and varies by regions. During the 2013/2014 season, the incidence rates of ILI in Chungju, Ulsan, and Seoul were greater than those from other two seasons due to the pandemic of influenza.
Karen Roderick completed her undergraduate studies at Hood College and works as a Research Associate for Lonza BioScience, Walkersville. Her focus is on the development of non-animal origin culture media for vaccine and therapeutic medicine.
Cell-based influenza vaccine production is quickly growing in the vaccine industry to meet the threat of pandemic outbreaks and to eliminate health concerns associated with egg protein allergies. Madin Darby Canine Kidney (MDCK) is a commonly used cell line for production of influenza virus and the cell-based manufacture of inactivated flu vaccines. Expansion of MDCK cells allows for rapid response and quick scale up compared to the traditional egg based vaccine manufacturing process. This study examines the feasibility of using microcarrier-based cell culture for the expansion of MDCK cells in 3D. Several media were compared for their ability to support MDCK cell growth in planar and suspension culture as well as their ability to support flu virus production from MDCK cells. ProMDCK (2D) and ProMDCK (3D) (Lonza) supported excellent cell proliferation and virus production in both planar culture and on multiple types of microcarriers. The preparation and usage of the various microcarriers were based on the manufactures’ recommendations and were evaluated for ease of use, ability to support cell proliferation and virus production, and ability to support MDCK expansion without cell dissociation in disposable culture systems.